CF release from native DPPC/DPPG-liposomes and POPC/POPG-liposomes as well as liposome complexes withPLL,37C close to the transition temperature that is about 40C.This indicates that the DPPC/DPPG-liposomes being in the solid state have an impermeable membrane and complexation with PLL,leading to single covered vesicles or vesicle aggregates,doesnot disturb the integrity of the liposome membrane.
However, if the native or PLL-covered DPPC/DPPG-liposomes were heated to 54 °C (lipids are transferred to the fluid state with an accompanying reduction in the vesicle size,as was previously described) a significant release is observed (profiles 6 and 7, respectively). We propose that the higher membrane permeability of the liposomes in the fluid state compared to the solid is due to the presence of defects in the fluid membrane or to a transient fragmentation that must occur during the vesicle size reduction. The observed different permeability is in agreement with studies on DPPC-based vesicle ion permeability in different phase states [39]. Our
experimentsreveal that PLL-covered“fluid”vesicles release the dyefaster,indicating a direct effect of bound PLL molecules on membrane permeability (Fig. 5A, profile 7). At the same time we suppose that PLL molecules do not translocate across lipid membrane because just short polypeptides are able to cross the membranes .
然而,如果本国或PLL-covered DPPC / DPPG-liposomes被加热到54°C(脂质会转移到流体状态与一个相应的减少小泡大小,正如以前描述)的一个重要的释放是观察(型材,分别6和7)。我们建议对高脂质体膜通透性的流体中固体状态相比,由于缺陷的存在流体膜或瞬态fragm...
从本地DPPC CF / DPPG-liposomes释放和POPC / POPG-liposomes以及微脂粒复合体,而且withPLL接近转变温度大约是40C。这表明,DPPG-liposomes DPPC处于/固态有一个不透水膜和络合和锁相环,导致覆盖或小泡囊泡单粒料、不干扰脂质体膜的完整性。
然而,如果本国或PLL-covered DPPC / DPPG-liposomes被加热到54°C
希望能帮到你
搞笑吧。。。。这么专业的文献让网友翻译。。。如果你是搞这一行的应该就能看懂啊。
Journal of Controlled Release
Volume 117, Issue 1, 22 January 2007, Pages 111-120
Coating of negatively charged liposomes by polylysine: Drug release study
Dmitry Volodkina, , , Helmuth Mohwaldb, Jean-Claude Voegela and Vincent Balla本回答被提问者采纳
然而,如果本国或PLL-covered DPPC / DPPG-liposomes被加热到54°C
然而，如果本地人或pll-covereddppc/dppg-liposomes被发热54°c源文件(脂类被转学到流态同一个陪同减少在囊大小，由于先前描述)一个重大版本被观(剖面6，7
experimentsreveal，pll-covered“流体”囊泡释放dyefaster，表明一个约束的直接影响锁相环分子在膜通透性(无花果。5a，剖面7)。同时我们假设锁相环分子不要易位横跨
