如题所述
æ¯åæ ¸è¡¨è¾¾è½½ä½pGEX-6p-1å§ã
pGEXç³»åçæµåºå¼ç©é½æ¯ä¸æ ·çï¼ä¸è¬çæµåºå ¬å¸æéç¨çpGEX-F/R ååæµåºå¼ç©ã
pGEX-Fï¼GGGCTGGCAAGCCACGTTTGGTG
pGEX-Rï¼CCGGGAGCTGCATGTGTCAGAGG
pGEXç³»åçæµåºå¼ç©é½æ¯ä¸æ ·çï¼ä¸è¬çæµåºå ¬å¸æéç¨çpGEX-F/R ååæµåºå¼ç©ã
pGEX-Fï¼GGGCTGGCAAGCCACGTTTGGTG
pGEX-Rï¼CCGGGAGCTGCATGTGTCAGAGG
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