柑桔木虱在健康和黄龙病柑桔树上的刺探电位图谱比较
柑桔黄龙病被视为柑桔害虫病里最严重的。这是一种细菌病,柑桔黄龙病可以危害到各种柑橘类水果,并且导致受感染果树在感染后几年内枯黄,萎缩,甚至死亡。亚洲柑桔木虱Diaphorina citri Kuwayama 隶同翅目Homoptera 木虱科Psyllidae,柑桔木虱是严重的柑橘害虫,也是传播柑桔黄龙病的主要传媒介体。
亚洲柑桔木虱属植食性刺吸式口器昆虫,其传毒方式与取食行为密切相关。本实验研究了柑桔木虱在健康和黄龙病柑橘树上的取食行为差异,以EPG(electrical penetration graph)技术为主,自由状态下的刺吸取食行为为辅,以刺探电位图谱为取食行为比较提供依据。
亚洲柑桔木虱在酸桔叶片上取食共产生8种不同的波形,主要由非刺探波(np波)、路径波(pathway phase)韧皮部分泌唾液波(E1波)、韧皮部被动吸食波(E2波)以及木质部主动吸食波(G波),此外,还发现在其他胸喙亚目刺吸式口器昆虫取食波中没有的D波,D波是柑桔木虱口针刺探筛管相关的波形,是亚洲柑桔木虱特有的刺探波形。
感病叶片表面革质化并未影响木虱对寄主植物的选择,木虱口针仍然能够在革质化的叶片上进行刺探活动,木虱口针在叶片表面停留的时间没有明显差异。
木虱口针刺入叶片表皮组织在薄壁组织组织穿刺寻找取食位点的过程中所用时间在中度发病和严重发病植株薄壁组织中的刺探波(路径波,C波)平均时间都显著长于健康组,并且随发病程度的增加而延长。
感病叶片薄壁组织结构的改变显著增加了木虱口针在叶肉组织中穿刺寻取食位点所需要的时间。
木虱口针首次到达韧皮部分泌唾液时间点和首次在韧皮部持续吸食时间点随发病程度的增加显著向后延迟。
木虱在感病叶片韧皮部取食变得越来越困难。
木虱在健树叶片上很少在木质部中取食,但在感病叶片上木虱在木质部中取食的比例和时间显著增加,发病后期木质部是木虱主要的取食部位。
The harmfulness is regarded as the most serious illness offersorange pests. This is a bacterial disease, harmfulness can harm to all sorts of citrus fruit, fruit and cause infection in a few years after infection, atrophy, and even death yellow. Asia Diaphorina lema citrus citri Kuwayama Li Homoptera wood lice eyes with wings, citrus wood lice Psyllidae secco is serious pest, citrus spread the harmfulness of main preach.
Asian citrus plant thorn of wood lice (type, the poisonous insect mouthparts and feeding behavior are closely related. The experimental research on the wood lice barbarions health and huanglong disease of oranges, feeding behavior to penetration electrical (EPG) technology, graph of free condition from food to spy on potential, behavior for feeding behavior comparison map.
Wood lice in Asian orange orange leaf feeding of acid produced eight different waveform, mainly composed of spying on the wave (np), pathway (phase) path wave wave (E1 saliva secretion phloem phloem passive smoking wave), wave (E2) and xylem active snorting wave (G), in addition, also found in other chest beak mysticetes thorn attracts insects in the mouth of feeding wave wave, D D without a citrus wood lice mouth needle spying on the screen of the waveform is related to the wood lice unique Asian citrus spying waveform.
Feeling sick leaves leathery change does not affect surface of wood lice host plants, wood lice in mouth needle of blade leathery on spying activities, wood lice mouth needle leaf in the surface of the time stay no obvious difference.
Wood lice needle leaf epidermis the mouth in the parenchyma organization for the process of feeding sites puncture in time in moderate and severe disease pathogenesis of spying on the waves were parenchyma, C (path) average time significantly longer health groups, and with the increase of the degree of disease and extension.
Feeling sick leaves parenchyma structure change significantly increased wood lice in mouth needle biopsy found in the mesophyll feeding sites.
Wood lice mouth needle first arrived phloem secretion and first time in salia suck time with continuous phloem significantly increased morbidity of backward delayed.
Wood lice in feeling sick leaves phloem feeding became more and more difficult.
Wood lice in rehabilitation leaves little in xylem on feeding, but in the sense of the disease in xylem in wood lice eat significantly increased the proportion of time, and the later is the primary xylem wood lice eat.
Asian citrus plant thorn of wood lice (type, the poisonous insect mouthparts and feeding behavior are closely related. The experimental research on the wood lice barbarions health and huanglong disease of oranges, feeding behavior to penetration electrical (EPG) technology, graph of free condition from food to spy on potential, behavior for feeding behavior comparison map.
Wood lice in Asian orange orange leaf feeding of acid produced eight different waveform, mainly composed of spying on the wave (np), pathway (phase) path wave wave (E1 saliva secretion phloem phloem passive smoking wave), wave (E2) and xylem active snorting wave (G), in addition, also found in other chest beak mysticetes thorn attracts insects in the mouth of feeding wave wave, D D without a citrus wood lice mouth needle spying on the screen of the waveform is related to the wood lice unique Asian citrus spying waveform.
Feeling sick leaves leathery change does not affect surface of wood lice host plants, wood lice in mouth needle of blade leathery on spying activities, wood lice mouth needle leaf in the surface of the time stay no obvious difference.
Wood lice needle leaf epidermis the mouth in the parenchyma organization for the process of feeding sites puncture in time in moderate and severe disease pathogenesis of spying on the waves were parenchyma, C (path) average time significantly longer health groups, and with the increase of the degree of disease and extension.
Feeling sick leaves parenchyma structure change significantly increased wood lice in mouth needle biopsy found in the mesophyll feeding sites.
Wood lice mouth needle first arrived phloem secretion and first time in salia suck time with continuous phloem significantly increased morbidity of backward delayed.
Wood lice in feeling sick leaves phloem feeding became more and more difficult.
Wood lice in rehabilitation leaves little in xylem on feeding, but in the sense of the disease in xylem in wood lice eat significantly increased the proportion of time, and the later is the primary xylem wood lice eat.