目的：
观察小鼠胸腺及脾细胞受照后p21基因表达的剂量效应关系。
方法：
用0.5、1、2、4、6 Gy X射线照射小鼠胸腺细胞及脾细胞，在照射后4 h和24 h，用实时定量PCR法对p21基因的表达变化进行检测，并用骨髓嗜多染网织红细胞微核检测做对比。
结果：
1．0～6 Gy X线照射4 h和24h，小鼠胸腺细胞和脾细胞p21基因的相对表达量均随剂量增大逐渐增加，并于6 Gy时达最高（P<0.05），但胸腺细胞1 Gy照射4h时相对表达量升高不显著（P>0.05）。
2．0～6 Gy X线照射后4 h和24 h，小鼠骨髓网织红细胞微核率均随剂量增加逐渐升高，6 Gy时达最大值（P<0.01），且照后24 h各剂量点微核率均略高于照后4 h。
结论：
对量效实验结果进行线性关系拟合， p21基因的剂量效应关系在其所研究的时间点有较理想的线性关系，得到较高的r值（r>0.98）和较小的P值(P<0.005)，具备了作为电离辐射生物标志物的条件。
关 键 词：电离辐射；生物标志物；p21
Objective:
Thymus and spleen cells in mice after irradiation the expression of p21 gene dosage effect relationship.
Method:
0.5,1,2,4,6 Gy X-ray irradiation with thymus cells and spleen cells 4 h after irradiation and 24 h, with real-time quantitative PCR, expression of the p21 gene were detected, and bone marrow stained with reticulocyte micronucleus test to do comparison.
Results:
1.0 ~ 6 Gy X-irradiation 4 h and 24h, mouse thymus cells and spleen cells in the relative expression of p21 gene increased with increasing dose increase, and reached to the peak at 6 Gy (P <0.05), but the thymus 1 Gy irradiated cells 4h increased when the relative expression level was not significant (P> 0.05).
2.0 ~ 6 Gy X rays after 4 h and 24 h, bone marrow reticulocyte micronucleus rate gradually increased with the dose, 6 Gy, with the maximum (P <0.01), and 24 h after irradiation The micronucleus rate slightly higher than the dose point after irradiation 4 h.
Conclusion:
The results of the linear dose-effect relationship between fit, p21 gene dose-response relationship of the time in which they have an ideal linear relationship, get a higher r value (r> 0.98) and smaller P values (P <0.005), with ionizing radiation as a biomarker conditions.
Key words: ionizing radiation; biological markers; p21
Thymus and spleen cells in mice after irradiation the expression of p21 gene dosage effect relationship.
Method:
0.5,1,2,4,6 Gy X-ray irradiation with thymus cells and spleen cells 4 h after irradiation and 24 h, with real-time quantitative PCR, expression of the p21 gene were detected, and bone marrow stained with reticulocyte micronucleus test to do comparison.
Results:
1.0 ~ 6 Gy X-irradiation 4 h and 24h, mouse thymus cells and spleen cells in the relative expression of p21 gene increased with increasing dose increase, and reached to the peak at 6 Gy (P <0.05), but the thymus 1 Gy irradiated cells 4h increased when the relative expression level was not significant (P> 0.05).
2.0 ~ 6 Gy X rays after 4 h and 24 h, bone marrow reticulocyte micronucleus rate gradually increased with the dose, 6 Gy, with the maximum (P <0.01), and 24 h after irradiation The micronucleus rate slightly higher than the dose point after irradiation 4 h.
Conclusion:
The results of the linear dose-effect relationship between fit, p21 gene dose-response relationship of the time in which they have an ideal linear relationship, get a higher r value (r> 0.98) and smaller P values (P <0.005), with ionizing radiation as a biomarker conditions.
Key words: ionizing radiation; biological markers; p21
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第1个回答 2010-05-27
Purpose:
Observe the thymus and spleen cells from mice, according to p21 after gene expression effect.
Methods:
Using 0.5, 1, 2, 4 and 6 Gy X ray cells and spleen cells in mice, the thymus gland in April 24 h after irradiation and real-time quantitatie polymerase chain reaction (PCR), p21 method of gene expression changes, and bone marrow or more red dye nets woven testing results.
Results:
1.0 ~ 6 Gy X-ray irradiation 4 h and 24h, mice thymus and spleen cells p21 cell gene expression with the relative quantity increases gradually increase dosage, and six of the highest when Gy (P < 0.05), but the thymus cell 1 Gy irradiation increased when the relative amount 4h expression are not significant (P > 0.05).
2 ~ 6 Gy X-ray irradiation and 24 h after 4 h, mouse bone marrow reticulocytosis micronucleus rate increased with increase dosage gradually, 6 Gy maximum when (P < 0.01), and after 24 h according to the dosage: micronucleus rate slightly above 4 h as.
Conclusion:
Experimental results of concentration-response linear fitting relationship, p21 gene effect in the study of the time have ideal linear relationship, have higher r value (r > 0.98) and small P value (P < 0.005), as a biomarker of ionizing radiation.
Key words: shut ionizing radiation, Biological marker, p21
Observe the thymus and spleen cells from mice, according to p21 after gene expression effect.
Methods:
Using 0.5, 1, 2, 4 and 6 Gy X ray cells and spleen cells in mice, the thymus gland in April 24 h after irradiation and real-time quantitatie polymerase chain reaction (PCR), p21 method of gene expression changes, and bone marrow or more red dye nets woven testing results.
Results:
1.0 ~ 6 Gy X-ray irradiation 4 h and 24h, mice thymus and spleen cells p21 cell gene expression with the relative quantity increases gradually increase dosage, and six of the highest when Gy (P < 0.05), but the thymus cell 1 Gy irradiation increased when the relative amount 4h expression are not significant (P > 0.05).
2 ~ 6 Gy X-ray irradiation and 24 h after 4 h, mouse bone marrow reticulocytosis micronucleus rate increased with increase dosage gradually, 6 Gy maximum when (P < 0.01), and after 24 h according to the dosage: micronucleus rate slightly above 4 h as.
Conclusion:
Experimental results of concentration-response linear fitting relationship, p21 gene effect in the study of the time have ideal linear relationship, have higher r value (r > 0.98) and small P value (P < 0.005), as a biomarker of ionizing radiation.
Key words: shut ionizing radiation, Biological marker, p21
第2个回答 2010-05-27
Purpose:
Observe the thymus and spleen cells from mice, according to p21 after gene expression effect.
Methods:
Using 0.5, 1, 2, 4 and 6 Gy X ray cells and spleen cells in mice, the thymus gland in April 24 h after irradiation and real-time quantitatie polymerase chain reaction (PCR), p21 method of gene expression changes, and bone marrow or more red dye nets woven testing results.
Results:
1.0 ~ 6 Gy X-ray irradiation 4 h and 24h, mice thymus and spleen cells p21 cell gene expression with the relative quantity increases gradually increase dosage, and six of the highest when Gy (P < 0.05), but the thymus cell 1 Gy irradiation increased when the relative amount 4h expression are not significant (P > 0.05).
2 ~ 6 Gy X-ray irradiation and 24 h after 4 h, mouse bone marrow reticulocytosis micronucleus rate increased with increase dosage gradually, 6 Gy maximum when (P < 0.01), and after 24 h according to the dosage: micronucleus rate slightly above 4 h as.
Conclusion:
Experimental results of concentration-response linear fitting relationship, p21 gene effect in the study of the time have ideal linear relationship, have higher r value (r > 0.98) and small P value (P < 0.005), as a biomarker of ionizing radiation.
Observe the thymus and spleen cells from mice, according to p21 after gene expression effect.
Methods:
Using 0.5, 1, 2, 4 and 6 Gy X ray cells and spleen cells in mice, the thymus gland in April 24 h after irradiation and real-time quantitatie polymerase chain reaction (PCR), p21 method of gene expression changes, and bone marrow or more red dye nets woven testing results.
Results:
1.0 ~ 6 Gy X-ray irradiation 4 h and 24h, mice thymus and spleen cells p21 cell gene expression with the relative quantity increases gradually increase dosage, and six of the highest when Gy (P < 0.05), but the thymus cell 1 Gy irradiation increased when the relative amount 4h expression are not significant (P > 0.05).
2 ~ 6 Gy X-ray irradiation and 24 h after 4 h, mouse bone marrow reticulocytosis micronucleus rate increased with increase dosage gradually, 6 Gy maximum when (P < 0.01), and after 24 h according to the dosage: micronucleus rate slightly above 4 h as.
Conclusion:
Experimental results of concentration-response linear fitting relationship, p21 gene effect in the study of the time have ideal linear relationship, have higher r value (r > 0.98) and small P value (P < 0.005), as a biomarker of ionizing radiation.
第3个回答 2010-05-27
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