L-色氨酸是人和动物生命活动中八种必须氨基酸之一，对人和动物的生长发育、新陈代谢起着重要作用。L-色氨酸的生产方法有多种，其中以发酵法最具发展前途。因此，如何采取有效措施提高发酵液中L-色氨酸的产率，并将其精制出来是目前急待解决的问题。本文通过对种树脂的筛选、吸附和洗脱条件的优化，以及对脱色、结晶方法的研究与摸索，最终确定强酸型阳离子树脂HZ-001在pH=3，3倍床体积流速，温度室温，对色氨酸有最大的吸附作用；将发酵液离心去沉淀并用活性炭脱色后通过HZ-001强酸型阳离子交换树脂柱，再用2mol/L的氨水6倍床体积流速在室温下进行洗脱，收集洗脱液，60℃浓缩为过饱和状态后加入30%无水乙醇，4℃下100rpm摇起晶20h，过滤搜集结晶并真空干燥得白色粉片状L-色氨酸晶体。总体收率为72.36%，纯度达97.4%。
L-tryptophan is a kind of necessary amino acid.It is useful for the growth of people and animals.There are several production methods of L-tryptophan,ultimately only L-tryptophan production by fermentation proved commercially feasible. Therefore,increasing the production and extraction of L-tryptophan are the problems that needed to be settled at present. Through a variety of resin filter, adsorption and elution conditions for the optimization, as well as decolorization, crystallization methods of research, this article eventually determine the strong acid cation resin HZ-001 which is at pH 3, 3BV / h, having the largest adsorption to the tryptophan; the fermentation broth were centrifuged to remove sediment,then treated with activated carbon to be decolored and then treated with strong acid cation resin HZ-001. After that,2mol/l amino acid were passed through the HZ-001 ion exchanging column,kept almost constants when the flow rate stayed 6 BV / h. At 60℃,it was condensed into the saturated state and then added Ethanol until its percentage reaching 30%. At 4 ℃ under 100rpm placed in the shaker 20h, the collection of L-tryptophan was dried up in vacuo. The total yield of L-tryptophan was 72.36% and purity was 97.4% in the process.
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L - tryptophan and animal life activities is one of eight kinds of amino acids, and must be on the growth and development of the animal, and plays an important role in metabolism. L - tryptophan production methods are various, including the most promising by fermentation. Therefore, how to take effective measures to improve the result of L - tryptophan yield and its refined out is the problem to be solved. Based on a kind of resin adsorption and elution requirement, the optimization, and the method of bleaching, crystallization and fumble, final acid type cationic resin in speed - 001 3,3-bis (pH = volume velocity and temperature of double bed room, to have the biggest tryptophan adsorption, Will result to precipitate and centrifugal by decolour activated speed - 001 acid type of cation exchange resin column, reoccupy 2mol/L ammonia six times in bed room under the volume velocity, collect eluent, with 60 degrees enrichment for supersaturated after joining 30% anhydrous alcohol, 4 degrees Celsius 100rpm under wave filter 20h wafer, collecting on crystallization and vacuum drying white powder flake L - tryptophan crystals. Overall, the purity of 72.36% yield of.
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第1个回答 2009-05-08
The L- tryptophan is the person and in the animal vital activity eight kinds must one of amino acids, to the person and animal's growth, metabolism is playing the influential role. The L- tryptophan's production method has many kinds, most has the career development by the zymotechnics. Therefore, how to adopt in the effective action enhancement fermentation fluid the L- tryptophan production rate, and purifies it is the present pending issue. This article through to plants the resin screening, the adsorption and the elution requirement optimization, as well as to the decolorization, the crystallization method's research and the fumble, is definite finally strong acid positive ion resin HZ-001 in the pH=3,3 time of bed volumetric flow rate, the temperature room temperature, has the biggest adsorption to the tryptophan; Will ferment the fluid offcenter to precipitate and decolorizes after the activated charcoal through the HZ-001 strong acid cation exchange resin column, uses 2mol/L the ammonia water 6 time of bed volumetric flow rate to carry on the elution again under the room temperature, the collection eluent, 60℃ the concentration add-on enters 30% absolute ethyl alcohol for the oversaturation condition, 4℃ under 100rpm swings graining 20h, the filtration collection crystallization and the vacuum drying much toner laminated shape L- tryptophan crystal. Overall receiving rate is 72.36%, the purity reaches 97.4%.
第2个回答 2009-05-09
L-色氨酸是人和动物生命活动中八种必须氨基酸之一，对人和动物的生长发育、新陈代谢起着重要作用。L-色氨酸的生产方法有多种，其中以发酵法最具发展前途。因此，如何采取有效措施提高发酵液中L-色氨酸的产率，并将其精制出来是目前急待解决的问题。本文通过对种树脂的筛选、吸附和洗脱条件的优化，以及对脱色、结晶方法的研究与摸索，最终确定强酸型阳离子树脂HZ-001在pH=3，3倍床体积流速，温度室温，对色氨酸有最大的吸附作用；将发酵液离心去沉淀并用活性炭脱色后通过HZ-001强酸型阳离子交换树脂柱，再用2mol/L的氨水6倍床体积流速在室温下进行洗脱，收集洗脱液，60℃浓缩为过饱和状态后加入30%无水乙醇，4℃下100rpm摇起晶20h，过滤搜集结晶并真空干燥得白色粉片状L-色氨酸晶体。总体收率为72.36%，纯度达97.4%。
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