如题所述
ATCCCRL-1772 C2C12
基本信息
资源编号 ATCCCRL-1772
资源名称 C2C12
种属 Mus musculus, mouse muscle
类型 myoblast
形态 myoblast
提供形式 frozen
安全等级 1
应用领域 transfection host
培养方法
培养基 The base medium for this cell line is ATCC-formulated Dulbecco's
Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth
medium, add the following components to the base medium: fetal bovine serum to a
final concentration of 10%.
传代方法 Protocol: IMPORTANT - DO NOT ALLOW CULTURES TO BECOME CONFLUENT.
Cultures must not be allowed to become confluent as this will deplete the
myoblastic population in the culture. Myotube formation is enhanced when the
medium is supplemented with 10% horse serum instead of fetal bovine serum. 1.
Remove and discard culture medium. 2.Briefly rinse the cell layer with 0.25%
(w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which
contains trypsin inhibitor. 3.Add 2.0 to 3.0 mL of Trypsin-EDTA solution to
flask and observe cells under an inverted microscope until cell layer is
dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not
agitate the cells by hitting or shaking the flask while waiting for the cells to
detach. Cells that are difficult to detach may be placed at 37°C to facilitate
dispersal. 4.Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by
gently pipetting. 5.Add appropriate aliquots of the cell suspension to new
culture vessels. Inoculate at a cell concentration between 1.5 X 10 5 and 1.0 X
10 6 viable cells/75 cm2. Corning® T-75 flasks (catalog #430641) are recommended
for subculturing this product. 6. Incubate cultures at 37°C.
Medium Renewal:
Every two to three days
生长条件 Temperature: 37.0°C
生长特性 adherent
存储条件 liquid nitrogen vapor phase;Freeze medium: Complete growth medium
supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor
phase
详细说明 （可见北纳生物）
描述 The C2C12 cell line differentiates rapidly, forming contractile
myotubes and producing characteristic muscle proteins.
Treatment with bone
morphogenic protein 2 (BMP-2) cause a shift in the differentiation pathway from
myoblastic to osteoblastic.
Tested and found negative for ectromelia virus
(mousepox).
Strain C3H
Derivation This is a subclone (produced by H. Blau, et al) of the mouse
myoblast cell line established by D. Yaffe and O. Saxel.
基本信息
资源编号 ATCCCRL-1772
资源名称 C2C12
种属 Mus musculus, mouse muscle
类型 myoblast
形态 myoblast
提供形式 frozen
安全等级 1
应用领域 transfection host
培养方法
培养基 The base medium for this cell line is ATCC-formulated Dulbecco's
Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth
medium, add the following components to the base medium: fetal bovine serum to a
final concentration of 10%.
传代方法 Protocol: IMPORTANT - DO NOT ALLOW CULTURES TO BECOME CONFLUENT.
Cultures must not be allowed to become confluent as this will deplete the
myoblastic population in the culture. Myotube formation is enhanced when the
medium is supplemented with 10% horse serum instead of fetal bovine serum. 1.
Remove and discard culture medium. 2.Briefly rinse the cell layer with 0.25%
(w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which
contains trypsin inhibitor. 3.Add 2.0 to 3.0 mL of Trypsin-EDTA solution to
flask and observe cells under an inverted microscope until cell layer is
dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not
agitate the cells by hitting or shaking the flask while waiting for the cells to
detach. Cells that are difficult to detach may be placed at 37°C to facilitate
dispersal. 4.Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by
gently pipetting. 5.Add appropriate aliquots of the cell suspension to new
culture vessels. Inoculate at a cell concentration between 1.5 X 10 5 and 1.0 X
10 6 viable cells/75 cm2. Corning® T-75 flasks (catalog #430641) are recommended
for subculturing this product. 6. Incubate cultures at 37°C.
Medium Renewal:
Every two to three days
生长条件 Temperature: 37.0°C
生长特性 adherent
存储条件 liquid nitrogen vapor phase;Freeze medium: Complete growth medium
supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor
phase
详细说明 （可见北纳生物）
描述 The C2C12 cell line differentiates rapidly, forming contractile
myotubes and producing characteristic muscle proteins.
Treatment with bone
morphogenic protein 2 (BMP-2) cause a shift in the differentiation pathway from
myoblastic to osteoblastic.
Tested and found negative for ectromelia virus
(mousepox).
Strain C3H
Derivation This is a subclone (produced by H. Blau, et al) of the mouse
myoblast cell line established by D. Yaffe and O. Saxel.
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